RNA-seq of isolated chromaffin cells highlights the role of sex-linked and imprinted genes in adrenal medulla development
- Author(s)
- Chan, WH; Komada, M; Fukushima, T; Southard-Smith, EM; Anderson, CR; Wakefield, MJ;
- Details
- Publication Year 2019-03-08,Volume 9,Issue #1,Page 3929
- Journal Title
- Scientific Reports
- Publication Type
- Journal Article
- Abstract
- Adrenal chromaffin cells and sympathetic neurons synthesize and release catecholamines, and both cell types are derived from neural crest precursors. However, they have different developmental histories, with sympathetic neurons derived directly from neural crest precursors while adrenal chromaffin cells arise from neural crest-derived cells that express Schwann cell markers. We have sought to identify the genes, including imprinted genes, which regulate the development of the two cell types in mice. We developed a method of separating the two cell types as early as E12.5, using differences in expression of enhanced yellow fluorescent protein driven from the tyrosine hydroxylase gene, and then used RNA sequencing to confirm the characteristic molecular signatures of the two cell types. We identified genes differentially expressed by adrenal chromaffin cells and sympathetic neurons. Deletion of a gene highly expressed by adrenal chromaffin cells, NIK-related kinase, a gene on the X-chromosome, results in reduced expression of adrenaline-synthesizing enzyme, phenyl-N-methyl transferase, by adrenal chromaffin cells and changes in cell cycle dynamics. Finally, many imprinted genes are up-regulated in chromaffin cells and may play key roles in their development.
- Research Division(s)
- Bioinformatics
- PubMed ID
- 30850723
- Publisher's Version
- https://doi.org/10.1038/s41598-019-40501-0
- Open Access at Publisher's Site
https://doi.org/10.1038/s41598-019-40501-0- NHMRC Grants
- NHMRC/1063822,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2019-03-13 11:53:59
Last Modified: 2019-03-13 11:56:50