The cis-regulatory atlas of the mouse immune system
Details
Publication Year 2019-02-07, Volume 176, Issue #4, Page 897-912 e20
Journal Title
Cell
Publication Type
Journal Article
Abstract
A complete chart of cis-regulatory elements and their dynamic activity is necessary to understand the transcriptional basis of differentiation and function of an organ system. We generated matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascades. This breadth of data enable variance components analysis that suggests that genes fall into two distinct classes, controlled by either enhancer- or promoter-driven logic, and multiple regression that connects genes to the enhancers that regulate them. Relating transcription factor (TF) expression to the genome-wide accessibility of their binding motifs classifies them as predominantly openers or closers of local chromatin accessibility, pinpointing specific cis-regulatory elements where binding of given TFs is likely functionally relevant, validated by chromatin immunoprecipitation sequencing (ChIP-seq). Overall, this cis-regulatory atlas provides a trove of information on transcriptional regulation through immune differentiation and a foundational scaffold to define key regulatory events throughout the immunological genome.
Publisher
Cell Press
WEHI Research Division(s)
Immunology
PubMed ID
30686579
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2019-03-13 08:04:21
Last Modified: 2019-03-13 10:01:27
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