Conformational switching of the pseudokinase domain promotes human MLKL tetramerization and cell death by necroptosis

Petrie, EJ; Sandow, JJ; Jacobsen, AV; Smith, BJ; Griffin, MDW; Lucet, IS; Dai, W; Young, SN; Tanzer, MC; Wardak, A; Liang, LY; Cowan, AD; Hildebrand, JM; Kersten, WJA; Lessene, G; Silke, J; Czabotar, PE; Webb, AI; Murphy, JM

Author(s): Petrie, EJ; Sandow, JJ; Jacobsen, AV; Smith, BJ; Griffin, MDW; Lucet, IS; Dai, W; Young, SN; Tanzer, MC; Wardak, A; Liang, LY; Cowan, AD; Hildebrand, JM; Kersten, WJA; Lessene, G; Silke, J; Czabotar, PE; Webb, AI; Murphy, JM;
Details: Publication Year 2018-06-21,Volume 9,Issue #1,Page 2422
Journal Title: Nature Communications
Publication Type: Journal Article
Abstract: Necroptotic cell death is mediated by the most terminal known effector of the pathway, MLKL. Precisely how phosphorylation of the MLKL pseudokinase domain activation loop by the upstream kinase, RIPK3, induces unmasking of the N-terminal executioner four-helix bundle (4HB) domain of MLKL, higher-order assemblies, and permeabilization of plasma membranes remains poorly understood. Here, we reveal the existence of a basal monomeric MLKL conformer present in human cells prior to exposure to a necroptotic stimulus. Following activation, toggling within the MLKL pseudokinase domain promotes 4HB domain disengagement from the pseudokinase domain alphaC helix and pseudocatalytic loop, to enable formation of a necroptosis-inducing tetramer. In contrast to mouse MLKL, substitution of RIPK3 substrate sites in the human MLKL pseudokinase domain completely abrogated necroptotic signaling. Therefore, while the pseudokinase domains of mouse and human MLKL function as molecular switches to control MLKL activation, the underlying mechanism differs between species.
Publisher: Springer Nature
Research Division(s): Chemical Biology; Structural Biology; Systems Biology And Personalised Medicine; Cell Signalling And Cell Death
PubMed ID: 29930286
Publisher's Version: https://doi.org/10.1038/s41467-018-04714-7
Open Access at Publisher's Site: https://doi.org/10.1038/s41467-018-04714-7
NHMRC Grants: NHMRC/1117089, NHMRC/1058190, NHMRC/1079700, NHMRC/1105754, NHMRC/1057905, NHMRC/1124735,
Terms of Use/Rights Notice: Refer to copyright notice on published article.
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Creation Date: 2018-06-27 09:13:26

Last Modified: 2018-06-27 09:31:33