PhagoSight: an open-source MATLAB® package for the analysis of fluorescent neutrophil and macrophage migration in a zebrafish model.
- Author(s)
- Henry, KM; Pase, L; Ramos-Lopez, CF; Lieschke, GJ; Renshaw, SA; Reyes-Aldasoro, CC;
- Details
- Publication Year 2013,Volume 8,Issue #8,Page e72636
- Journal Title
- PLoS One
- Publication Type
- Journal Article
- Abstract
- Neutrophil migration in zebrafish larvae is increasingly used as a model to study the response of these leukocytes to different determinants of the cellular inflammatory response. However, it remains challenging to extract comprehensive information describing the behaviour of neutrophils from the multi-dimensional data sets acquired with widefield or confocal microscopes. Here, we describe PhagoSight, an open-source software package for the segmentation, tracking and visualisation of migrating phagocytes in three dimensions. The algorithms in PhagoSight extract a large number of measurements that summarise the behaviour of neutrophils, but that could potentially be applied to any moving fluorescent cells. To derive a useful panel of variables quantifying aspects of neutrophil migratory behaviour, and to demonstrate the utility of PhagoSight, we evaluated changes in the volume of migrating neutrophils. Cell volume increased as neutrophils migrated towards the wound region of injured zebrafish. PhagoSight is openly available as MATLAB® m-files under the GNU General Public License. Synthetic data sets and a comprehensive user manual are available from http://www.phagosight.org.
- Publisher
- Public Library of Science
- Research Division(s)
- Cancer And Haematology
- Publisher's Version
- https://doi.org/10.1371/journal.pone.0072636
- Open Access at Publisher's Site
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0072636- Terms of Use/Rights Notice
- Copyright: © 2013 Henry et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Creation Date: 2013-01-01 12:00:00