Flow cytometric analysis of STAT phosphorylation.
- Author(s)
- Murphy, J; Goldberg, GL;
- Journal Title
- Methods in Molecular Biology
- Publication Type
- Journal Article
- Abstract
- Flow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to the phosphorylated form of the STAT protein. This allows the tyrosine phosphorylation of a single STAT to be studied within a heterogeneous cell population and/or the phosphorylation of several STATs within the one cell type.
- Publisher
- Springer
- Keywords
- STAT phosphorylation ; Flow cytometry ; Phospho-flow
- Research Division(s)
- Inflammation
- Publisher's Version
- https://doi.org/10.1007/978-1-62703-242-1_11
- Terms of Use/Rights Notice
- © Springer, Part of Springer Science+Business Media
Creation Date: 2013-01-01 12:00:00