Analysis of Suppressor of Cytokine Signalling (SOCS) gene expression by real-time quantitative PCR.
- Author(s)
- Kolesnik, TB; Nicholson, SE;
- Journal Title
- Methods in Molecular Biology
- Publication Type
- Journal Article
- Abstract
- The Suppressor of Cytokine Signalling (SOCS) proteins are a family of negative regulators characterized by a central SH2 domain and C-terminal SOCS box motif. Cytokine Inducible SH2-containing protein (CIS), SOCS1, 2 and 3 are rapidly upregulated in response to cytokine stimulation and act to inhibit JAK/STAT signalling by a variety of mechanisms. The expression of SOCS proteins provides a level of specificity in the control of signalling, with SOCS proteins differentially upregulated in response to individual cytokines and in various cell-types. Real-time reverse transcription (RT) quantitative polymerase chain reaction (RT-qPCR) is an established technique for quantifying mRNA in biological samples, measuring the relative expression of genes of interest and identifying single nucleotide polymorphisms. Here we describe the use of SYBR(®) Green I RT-qPCR to quantify the relative expression level of SOCS mRNA in murine bone marrow-derived macrophages (BMDM). The approach can be universally applied to different cell types and various tissues.
- Publisher
- Springer
- Keywords
- suppressors of cytokine signalling ; SOCS ; murine bone marrow-derived macrophages (BMDM)
- Research Division(s)
- Inflammation
- Publisher's Version
- https://doi.org/10.1007/978-1-62703-242-1_17
- NHMRC Grants
- NHMRC/461219,
- Terms of Use/Rights Notice
- © Springer, Part of Springer Science+Business Media
Creation Date: 2013-01-01 12:00:00