Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery
- Author(s)
- Penno, M; Ernst, M; Hoffmann, P;
- Journal Title
- Methods in molecular biology
- Publication Type
- Journal Article
- Abstract
- Serum is unarguably the most used diagnostic fluid. As it circulates throughout the body, leakage peptides/proteins from damaged and dying cells, host-response proteins including inflammatory mediators, and aberrant secretions from tumors and diseased tissues are released into serum, potentially providing a rich source of disease biomarkers. Here, a method for extending access to the serum proteome by removing highly abundant proteins prior to comparative two-dimensional difference gel electrophoresis (2D DIGE) and subsequent protein digestion for identification by mass spectrometry is described.
- Research Division(s)
- Cell Signalling And Cell Death
- Publisher's Version
- https://doi.org/10.1007/978-1-61779-573-2_15
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2014-01-16 04:36:07