Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion
Details
Publication Year 2013-09, Volume 15, Issue #9, Page 1457-1472
Journal Title
CELLULAR MICROBIOLOGY
Publication Type
Journal Article
Abstract
Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria disease. To date, efforts to understand cellular events underpinning entry have been limited to insights from non-human parasites, with no studies at sub-micrometer resolution undertaken using the most virulent human malaria parasite, Plasmodium falciparum. This leaves our understanding of the dynamics of merozoite sub-cellular compartments during infection incomplete, in particular that of the secretory organelles. Using advances in P. falciparum merozoite isolation and new imaging techniques we present a three-dimensional study of invasion using electron microscopy, cryo-electron tomography and cryo-X-ray tomography. We describe the core architectural features of invasion and identify fusion between rhoptries at the commencement of invasion as a hitherto overlooked event that likely provides a critical step that initiates entry. Given the centrality of merozoite organelle proteins to vaccine development, these insights provide a mechanistic framework to understand therapeutic strategies targeted towards the cellular events of invasion.
Publisher
WILEY-BLACKWELL
Keywords
RED-BLOOD-CELLS; MALARIA PARASITE INVASION; TOXOPLASMA-GONDII; APICOMPLEXAN PARASITES; INFECTED ERYTHROCYTES; FINE STRUCTURE; SUBPELLICULAR MICROTUBULES; RHOPTRY PROTEINS; SURFACE-ANTIGEN; DENSE GRANULES
WEHI Research Division(s)
Infection And Immunity
Publisher's Version
https://doi.org/10.1111/cmi.12132
Rights Notice
© 2013 John Wiley & Sons Ltd


Creation Date: 2013-09-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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