CD154 CD4 T-cell dependence for effective memory influenza virus-specific CD8 T-cell responses
- Author(s)
- Olson, MR; Seah, SG; Edenborough, K; Doherty, PC; Lew, AM; Turner, SJ;
- Details
- Publication Year 2014-04-29,Volume 97,Issue #7,Page 605-11
- Journal Title
- Immunol Cell Biol
- Publication Type
- Journal Article
- Abstract
- CD40-CD154 (CD40 ligand) interactions are essential for the efficient priming of CD8+ cytotoxic T lymphocyte (CTL) responses. This is typically via CD4+CD154+ T-cell-dependent 'licensing' of CD40+ dendritic cells (DCs); however, DCs infected with influenza A virus (IAV) upregulate CD154 expression, thus enabling efficient CTL priming in the absence of CD4+ T activation. Therefore, it is unclear whether CD4 T cells and DCs have redundant or unique roles in the priming of primary and secondary CTL responses after infection. Here we determine the precise cellular interactions involved in CD40-CD154 regulation of both primary and secondary IAV-specific CTL responses. Infection of both CD40 KO and CD154 KO mice resulted in diminished quantitative and qualitative CTL responses after both primary and secondary infection. Adoptive transfer of CD154+, but not CD154 KO, CD4 T cells into CD154 KO mice restored both primary and secondary IAV-specific CD8 T-cell responses. These data show that, although CD154 expression on CD4 T cells and other cell types (that is, DCs) may be redundant for the priming of primary CTL responses, CD154 expression by CD4 T cells is required for the priming memory CD8 T cells that are capable of fully responding to secondary infection.Immunology and Cell Biology advance online publication, 29 April 2014; doi:10.1038/icb.2014.28.
- Publisher
- NPG
- Research Division(s)
- Immunology
- Publisher's Version
- https://doi.org/10.1038/icb.2014.28
- NHMRC Grants
- NHMRC/1037321, NHMRC/1043414,
- Terms of Use/Rights Notice
- © 2014 Australasian Society for Immunology
Creation Date: 2014-05-20 08:08:54
Last Modified: 2015-04-02 08:55:30