CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria
- Author(s)
- Lau, LS; Fernandez-Ruiz, D; Mollard, V; Sturm, A; Neller, MA; Cozijnsen, A; Gregory, JL; Davey, GM; Jones, CM; Lin, YH; Haque, A; Engwerda, CR; Nie, CQ; Hansen, DS; Murphy, KM; Papenfuss, AT; Miles, JJ; Burrows, SR; de Koning-Ward, T; McFadden, GI; Carbone, FR; Crabb, BS; Heath, WR;
- Details
- Publication Year 2014-05,Volume 10,Issue #5,Page e1004135
- Journal Title
- PLoS Pathogens
- Publication Type
- Journal Article
- Abstract
- To follow the fate of CD8+ T cells responsive to Plasmodium berghei ANKA (PbA) infection, we generated an MHC I-restricted TCR transgenic mouse line against this pathogen. T cells from this line, termed PbT-I T cells, were able to respond to blood-stage infection by PbA and two other rodent malaria species, P. yoelii XNL and P. chabaudi AS. These PbT-I T cells were also able to respond to sporozoites and to protect mice from liver-stage infection. Examination of the requirements for priming after intravenous administration of irradiated sporozoites, an effective vaccination approach, showed that the spleen rather than the liver was the main site of priming and that responses depended on CD8alpha+ dendritic cells. Importantly, sequential exposure to irradiated sporozoites followed two days later by blood-stage infection led to augmented PbT-I T cell expansion. These findings indicate that PbT-I T cells are a highly versatile tool for studying multiple stages and species of rodent malaria and suggest that cross-stage reactive CD8+ T cells may be utilized in liver-stage vaccine design to enable boosting by blood-stage infections.
- Publisher
- Public Library of Science
- Research Division(s)
- Infection And Immunity; Bioinformatics
- Publisher's Version
- https://doi.org/10.1371/journal.ppat.1004135
- Open Access at Publisher's Site
http://www.plospathogens.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.ppat.1004135- Terms of Use/Rights Notice
- Copyright: © 2014 Lau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creation Date: 2014-05-26 08:08:15