Stretched cell cycle model for proliferating lymphocytes
Details
Publication Year 2014-04-29,Volume 111,Issue #17,Page 6377-82
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Publication Type
Journal Article
Abstract
Stochastic variation in cell cycle time is a consistent feature of otherwise similar cells within a growing population. Classic studies concluded that the bulk of the variation occurs in the G1 phase, and many mathematical models assume a constant time for traversing the S/G2/M phases. By direct observation of transgenic fluorescent fusion proteins that report the onset of S phase, we establish that dividing B and T lymphocytes spend a near-fixed proportion of total division time in S/G2/M phases, and this proportion is correlated between sibling cells. This result is inconsistent with models that assume independent times for consecutive phases. Instead, we propose a stretching model for dividing lymphocytes where all parts of the cell cycle are proportional to total division time. Data fitting based on a stretched cell cycle model can significantly improve estimates of cell cycle parameters drawn from DNA labeling data used to monitor immune cell dynamics.
Publisher
National Academy of Sciences
Research Division(s)
Immunology
Open Access at Publisher's Site
http://www.pnas.org/content/111/17/6377.long
Terms of Use/Rights Notice
Freely available online through the PNAS open access option.


Creation Date: 2014-05-09 08:58:34
An error has occurred. This application may no longer respond until reloaded. Reload 🗙