Stretched cell cycle model for proliferating lymphocytes
- Author(s)
- Dowling, MR; Kan, A; Heinzel, S; Zhou, JH; Marchingo, JM; Wellard, CJ; Markham, JF; Hodgkin, PD;
- Details
- Publication Year 2014-04-29,Volume 111,Issue #17,Page 6377-82
- Journal Title
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type
- Journal Article
- Abstract
- Stochastic variation in cell cycle time is a consistent feature of otherwise similar cells within a growing population. Classic studies concluded that the bulk of the variation occurs in the G1 phase, and many mathematical models assume a constant time for traversing the S/G2/M phases. By direct observation of transgenic fluorescent fusion proteins that report the onset of S phase, we establish that dividing B and T lymphocytes spend a near-fixed proportion of total division time in S/G2/M phases, and this proportion is correlated between sibling cells. This result is inconsistent with models that assume independent times for consecutive phases. Instead, we propose a stretching model for dividing lymphocytes where all parts of the cell cycle are proportional to total division time. Data fitting based on a stretched cell cycle model can significantly improve estimates of cell cycle parameters drawn from DNA labeling data used to monitor immune cell dynamics.
- Publisher
- National Academy of Sciences
- Research Division(s)
- Immunology
- Publisher's Version
- https://doi.org/10.1073/pnas.1322420111
- Open Access at Publisher's Site
http://www.pnas.org/content/111/17/6377.long- Terms of Use/Rights Notice
- Freely available online through the PNAS open access option.
Creation Date: 2014-05-09 08:58:34