Proteome Informatics Research Group (iPRG)_2012: A study on detecting modified peptides in a complex mixture
- Author(s)
- Chalkley, RJ; Bandeira, N; Chambers, MC; Clauser, KR; Cottrell, JS; Deutsch, EW; Kapp, EA; Lam, HHN; McDonald, WH; Neubert, TA; Sun, RX;
- Details
- Publication Year 2014-01,Volume 13,Issue #1,Page 360-371
- Journal Title
- Molecular & Cellular Proteomics
- Publication Type
- Journal Article
- Abstract
- The proteome informatics research group of the Association of Biomolecular Resource Facilities conducted a study to assess the community's ability to detect and characterize peptides bearing a range of biologically occurring post-translational modifications when present in a complex peptide background. A data set derived from a mixture of synthetic peptides with biologically occurring modifications combined with a yeast whole cell lysate as background was distributed to a large group of researchers and their results were collectively analyzed. The results from the twenty-four participants, who represented a broad spectrum of experience levels with this type of data analysis, produced several important observations. First, there is significantly more variability in the ability to assess whether a results is significant than there is to determine the correct answer. Second, labile post-translational modifications, particularly tyrosine sulfation, present a challenge for most researchers. Finally, for modification site localization there are many tools being employed, but researchers are currently unsure of the reliability of the results these programs are producing.
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY
- Research Division(s)
- Systems Biology And Personalised Medicine
- Publisher's Version
- https://doi.org/10.1074/mcp.M113.032813
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2014-04-30 02:47:49
Last Modified: 2015-09-04 12:01:30