Co-sedimentation Assay for the Detection of Direct Binding to F-actin.
Journal Title
Bio-protocol
Publication Type
Journal Article
Abstract
This protocol describes measurement of direct protein-protein interactions by actin co-sedimentation assay. The actin co-sedimentation assay is a well-established technique and has been commonly used to demonstrate binding of proteins that interact directly with actin filaments 1, 2, 3, 4. We and others have previously shown that the damaged cell-recognition molecule C-type lectin 9A (Clec9A) recognises a conserved component within nucleated and non-nucleated cells that is exposed only when the cell membrane is damaged 5, 6. Here we use Clec9A as an example and present a detailed procedure for demonstrating the direct binding of purified recombinant Clec9A ectodomain to actin filaments
Publisher
bio-protocol
WEHI Research Division(s)
Cancer And Haematology; Immunology
Rights Notice
© Copyright www.bio-protocol.org


Creation Date: 2014-01-24 12:02:24
Last Modified: 0001-01-01 12:00:00
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