Germinal center-independent, IgM-mediated autoimmunity in sanroque mice lacking Obf1
Details
Publication Year 2014-01, Volume 92, Issue #1, Page 12-9
Journal Title
Immunology and cell biology
Publication Type
Journal Article
Abstract
Mice homozygous for a point mutation in the Rc3h1 gene encoding Roquin1, designated sanroque mice, develop a severe antibody-mediated autoimmune condition. The disease is T-cell intrinsic, exacerbated by macrophage-intrinsic defects and driven by excessive T follicular helper cell generation and spontaneous germinal centre (GC) formation. This culminates in abnormally high numbers of plasma cells secreting high-affinity autoreactive immunoglobulin G (IgG). Obf1 is a transcriptional co-activator required for normal T-cell-dependent antibody responses, and it is essential for GC formation under all circumstances so far tested. We crossed sanroque mice with Obf1-null mice to determine whether the hyperactivity of sanroque T cells could drive Obf1(-/-) B cells to differentiate to GC B cells, or conversely, if Obf1 loss would prevent sanroque-mediated autoimmune disease. Surprisingly, while sanroque/Obf1(-/-) mice did not form GC, they still developed autoimmune disease and succumbed even more rapidly than did sanroque mice. The disease was mediated by autoreactive IgM, which may have been derived from a pre-existing population of autoreactive B cells in the Obf1(-/-) mice responding to the over-exuberant activity of sanroque CD4 cells.
Publisher
NATURE PUBLISHING GROUP
Keywords
autoantibody; Obf1; sanroque
WEHI Research Division(s)
Molecular Immunology
NHMRC Grants
NHMRC/637306 NHMRC/575500
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2014-02-18 03:39:52
Last Modified: 2015-04-02 08:18:04
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