The search for RNA-binding proteins: a technical and interdisciplinary challenge
- Author(s)
- Smith, JM; Sandow, JJ; Webb, AI;
- Details
- Publication Year 2021-01-25,Volume 49,Issue #1,Page 393-403
- Journal Title
- Biochemical Society Transactions
- Abstract
- RNA-binding proteins are customarily regarded as important facilitators of gene expression. In recent years, RNA-protein interactions have also emerged as a pervasive force in the regulation of homeostasis. The compendium of proteins with provable RNA-binding function has swelled from the hundreds to the thousands astride the partnership of mass spectrometry-based proteomics and RNA sequencing. At the foundation of these advances is the adaptation of RNA-centric capture methods that can extract bound protein that has been cross-linked in its native environment. These methods reveal snapshots in time displaying an extensive network of regulation and a wealth of data that can be used for both the discovery of RNA-binding function and the molecular interfaces at which these interactions occur. This review will focus on the impact of these developments on our broader perception of post-transcriptional regulation, and how the technical features of current capture methods, as applied in mammalian systems, create a challenging medium for interpretation by systems biologists and target validation by experimental researchers.
- Publisher
- Portland Press
- Keywords
- Rna; RNA-binding proteins; biochemical techniques and resources
- Research Division(s)
- Advanced Technology And Biology
- PubMed ID
- 33492363
- Publisher's Version
- https://doi.org/10.1042/bst20200688
- Open Access at Publisher's Site
https://doi.org/10.1042/bst20200688- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2021-03-09 08:05:53
Last Modified: 2021-03-09 08:49:37