Kinetics of the SARS-CoV-2 antibody response and serological estimation of time since infection
- Author(s)
- Pelleau, S; Woudenberg, T; Rosado, J; Donnadieu, F; Garcia, L; Obadia, T; Gardais, S; Elgharbawy, Y; Velay, A; Gonzalez, M; Nizou, JY; Khelil, N; Zannis, K; Cockram, C; Merkling, SH; Meola, A; Kerneis, S; Terrier, B; de Seze, J; Planas, D; Schwartz, O; Dejardin, F; Petres, S; von Platen, C; Pellerin, SF; Arowas, L; de Facci, LP; Duffy, D; Cheallaigh, CN; Dunne, J; Conlon, N; Townsend, L; Duong, V; Auerswald, H; Pinaud, L; Tondeur, L; Backovic, M; Hoen, B; Fontanet, A; Mueller, I; Fafi-Kremer, S; Bruel, T; White, M;
- Journal Title
- Journal of Infectious Diseases
- Publication Type
- epub ahead of print
- Abstract
- BACKGROUND: Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a complex antibody response that varies by orders of magnitude between individuals and over time. METHODS: We developed a multiplex serological test for measuring antibodies to five SARS-CoV-2 antigens and the Spike proteins of seasonal coronaviruses. We measured antibody responses in cohorts of hospitalized patients and healthcare workers followed for up to eleven months after symptoms. A mathematical model of antibody kinetics was used to quantify the duration of antibody responses. Antibody response data were used to train algorithms for estimating time since infection. RESULTS: One year after symptoms, we estimate that 36% (95% range: 11%, 94%) of anti-Spike IgG remains, 31% (9%, 89%) anti-RBD IgG remains, and 7% (1%, 31%) anti-Nucleocapsid IgG remains. The multiplex assay classified previous infections into time intervals of 0-3 months, 3-6 months, and 6-12 months. This method was validated using data from a sero-prevalence survey in France, demonstrating that historical SARS-CoV-2 transmission can be reconstructed using samples from a single survey. CONCLUSIONS: In addition to diagnosing previous SARS-CoV-2 infection, multiplex serological assays can estimate the time since infection which can be used to reconstruct past epidemics.
- Publisher
- Oxford Academic
- Keywords
- SARS-SoV-2; antibody kinetics; sero-prevalence; surveillance; time since infection
- Research Division(s)
- Population Health And Immunity
- PubMed ID
- 34282461
- Publisher's Version
- https://doi.org/10.1093/infdis/jiab375
- Open Access at Publisher's Site
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Creation Date: 2021-08-16 10:40:52
Last Modified: 2021-08-16 11:17:57