Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
Details
Publication Year 2021-09-13, Volume 4, Issue #1, Page 1067
Journal Title
Communications Biology
Abstract
Cell embedment into a solid support matrix is considered essential for the culture of intestinal epithelial organoids and tumoroids, but this technique presents challenges that impede scalable culture expansion, experimental manipulation, high-throughput screening and diagnostic applications. We have developed a low-viscosity matrix (LVM) suspension culture method that enables efficient establishment and propagation of organoids and tumoroids from the human large intestine. Organoids and tumoroids cultured in LVM suspension recapitulate the morphological development observed in solid matrices, with tumoroids reflecting the histological features and genetic heterogeneity of primary colorectal cancers. We demonstrate the utility of LVM suspension culture for organoid and tumoroid bioreactor applications and biobanking, as well as tumoroid high-throughput drug sensitivity testing. These methods provide opportunities for the study and use of patient-derived organoids and tumoroids from the large intestine.
Publisher
NPG
WEHI Research Division(s)
Personalised Oncology; Bioinformatics
PubMed ID
34518628
Open Access at Publisher's Site
https://doi.org/10.1038/s42003-021-02607-y.
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2021-09-17 11:23:11
Last Modified: 2021-09-17 12:06:28
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