Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand

Orb&#225;n, &#193;; Longley, RJ; Sripoorote, P; Maneechai, N; Nguitragool, W; Butykai, &#193;; Mueller, I; Sattabongkot, J; Karl, S; K&#233;zsm&#225;rki, I

Author(s): Orbán, Á; Longley, RJ; Sripoorote, P; Maneechai, N; Nguitragool, W; Butykai, Á; Mueller, I; Sattabongkot, J; Karl, S; Kézsmárki, I;
Details: Publication Year 2021-09-17,Volume 11,Issue #1,Page 18547
Journal Title: Scientific Reports
Abstract: The rotating-crystal magneto-optical detection (RMOD) method has been developed for the rapid and quantitative diagnosis of malaria and tested systematically on various malaria infection models. Very recently, an extended field trial in a high-transmission region of Papua New Guinea demonstrated its great potential for detecting malaria infections, in particular Plasmodium vivax. In the present small-scale field test, carried out in a low-transmission area of Thailand, RMOD confirmed malaria in all samples found to be infected with Plasmodium vivax by microscopy, our reference method. Moreover, the magneto-optical signal for this sample set was typically 1-3 orders of magnitude higher than the cut-off value of RMOD determined on uninfected samples. Based on the serial dilution of the original patient samples, we expect that the method can detect Plasmodium vivax malaria in blood samples with parasite densities as low as [Formula: see text]5-10 parasites per microliter, a limit around the pyrogenic threshold of the infection. In addition, by investigating the correlation between the magnitude of the magneto-optical signal, the parasite density and the erythrocytic stage distribution, we estimate the relative hemozoin production rates of the ring and the trophozoite stages of in vivo Plasmodium vivax infections.
Research Division(s): Population Health And Immunity
PubMed ID: 34535695
Publisher's Version: https://doi.org/10.1038/s41598-021-97532-9
Open Access at Publisher's Site: https://doi.org/10.1038/s41598-021-97532-9
Terms of Use/Rights Notice: Refer to copyright notice on published article.
Documents: Orban A et al_Scientific Reports_2021.pdf - (1.78MB, application/pdf)

Creation Date: 2021-10-12 07:30:12

Last Modified: 2021-10-19 11:16:28