Regulation of the EphA2 receptor intracellular region by phosphomimetic negative charges in the kinase-SAM linker
Details
Publication Year 2021-12-02, Volume 12, Issue #1, Page 7047
Journal Title
Nature Communications
Abstract
Eph receptor tyrosine kinases play a key role in cell-cell communication. Lack of structural information on the entire multi-domain intracellular region of any Eph receptor has hindered understanding of their signaling mechanisms. Here, we use integrative structural biology to investigate the structure and dynamics of the EphA2 intracellular region. EphA2 promotes cancer malignancy through a poorly understood non-canonical form of signaling involving serine/threonine phosphorylation of the linker connecting its kinase and SAM domains. We show that accumulation of multiple linker negative charges, mimicking phosphorylation, induces cooperative changes in the EphA2 intracellular region from more closed to more extended conformations and perturbs the EphA2 juxtamembrane segment and kinase domain. In cells, linker negative charges promote EphA2 oligomerization. We also identify multiple kinases catalyzing linker phosphorylation. Our findings suggest multiple effects of linker phosphorylation on EphA2 signaling and imply that coordination of different kinases is necessary to promote EphA2 non-canonical signaling.
Publisher
NPG
WEHI Research Division(s)
Ubiquitin Signalling; Inflammation
PubMed ID
34857764
Open Access at Publisher's Site
https://doi.org/10.1038/s41467-021-27343-z
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2021-12-07 12:04:06
Last Modified: 2021-12-07 01:20:50
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