In Situ HiC
- Author(s)
- Johanson, TM; Allan, RS;
- Journal Title
- Methods in Moleculr BIology
- Abstract
- In situ HiC uses the relative frequency of DNA-DNA ligation events to reconstruct the three-dimensional architecture of a genome. As such, restriction enzyme digested ends of genomic DNA within fixed nuclei are tagged with biotinylated dNTPs. DNA-DNA ligation events generated via proximity ligation are then captured, amplified and next generation sequenced to determine their linear genomic position, but also their three-dimensional relationship. Here, we describe these steps in detail.
- Publisher
- Humana
- Research Division(s)
- Immunology
- PubMed ID
- 35103976
- Publisher's Version
- https://doi.org/10.1007/978-1-0716-2140-0_18
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2022-02-18 10:38:02
Last Modified: 2022-02-18 11:10:50