Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles
- Author(s)
- Tan, HX; Juno, JA; Esterbauer, R; Kelly, HG; Wragg, KM; Konstandopoulos, P; Alcantara, S; Alvarado, C; Jones, R; Starkey, G; Wang, BZ; Yoshino, O; Tiang, T; Grayson, ML; Opdam, H; D'Costa, R; Vago, A; Mackay, LK; Gordon, CL; Masopust, D; Groom, JR; Kent, SJ; Wheatley, AK;
- Details
- Publication Year 2022-01-28,Volume 7,Issue #67,Page eabf5314
- Journal Title
- Science Immunology
- Abstract
- Recent studies have established that memory B cells, largely thought to be circulatory in the blood, can take up long-term residency in inflamed tissues, analogous to widely described tissue-resident T cells. The dynamics of recruitment and retention of memory B cells to tissues and their immunological purpose remains unclear. Here, we characterized tissue-resident memory B cells (B(RM)) that are stably maintained in the lungs of mice after pulmonary influenza infection. Influenza-specific B(RM) were localized within inducible bronchus-associated lymphoid tissues (iBALTs) and displayed transcriptional signatures distinct from classical memory B cells in the blood or spleen while showing partial overlap with memory B cells in lung-draining lymph nodes. We identified lung-resident markers, including elevated expression of CXCR3, CCR6, and CD69, on hemagglutinin (HA)- and nucleoprotein (NP)-specific lung B(RM). We found that CCR6 facilitates increased recruitment and/or retention of B(RM) in lungs and differentiation into antibody-secreting cells upon recall. Although expression of CXCR3 and CCR6 was comparable in total and influenza-specific memory B cells isolated across tissues of human donors, CD69 expression was higher in memory B cells from lung and draining lymph nodes of human organ donors relative to splenic and PBMC-derived populations, indicating that mechanisms underpinning B(RM) localization may be evolutionarily conserved. Last, we demonstrate that human memory B cells in lungs are transcriptionally distinct to populations in lung-draining lymph nodes or PBMCs. These data suggest that B(RM) may constitute a discrete component of B cell immunity, positioned at the lung mucosa for rapid humoral response against respiratory viral infections.
- Research Division(s)
- Immunology
- PubMed ID
- 35089815
- Publisher's Version
- https://doi.org/10.1126/sciimmunol.abf5314
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- Refer to copyright notice on published article.
Creation Date: 2022-02-18 11:34:08
Last Modified: 2022-02-18 02:52:41