CyTOF mass cytometry analysis of human memory CD4(+) T cells and memory B cells
Details
Publication Year 2022-06-17,Volume 3,Issue #2,Page 101269
Journal Title
STAR Protocols
Abstract
High-dimensional mass cytometry provides unparalleled insight into the cellular composition of the immune system. Here, we describe a mass-cytometry-based protocol to examine memory CD4(+) T cell and memory B cell (MBC) responses in human peripheral blood. This approach allows for the identification of >50 distinct memory CD4(+) T cell and MBC populations from a single clinical sample. This highly reproducible protocol has been successfully applied to multiple infectious disease settings to identify correlates of susceptibility or protection from infection. For complete details on the use and execution of this protocol, please refer to Ioannidis et al. (2021).
Publisher
Elsevier
Keywords
Cell Biology; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology; Systems biology
WEHI Research Division(s)
Infectious Diseases And Immune Defence
PubMed ID
35378884
Open Access at Publisher's Site
https://doi.org/10.1016/j.xpro.2022.101269
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2022-04-08 10:53:26
Last Modified: 2022-04-08 11:17:07
An error has occurred. This application may no longer respond until reloaded. Reload 🗙