CyTOF mass cytometry analysis of human memory CD4(+) T cells and memory B cells
- Author(s)
- Ioannidis, LJ; Mitchell, AJ; Zheng, T; Hansen, DS;
- Details
- Publication Year 2022-06-17,Volume 3,Issue #2,Page 101269
- Journal Title
- STAR Protocols
- Abstract
- High-dimensional mass cytometry provides unparalleled insight into the cellular composition of the immune system. Here, we describe a mass-cytometry-based protocol to examine memory CD4(+) T cell and memory B cell (MBC) responses in human peripheral blood. This approach allows for the identification of >50 distinct memory CD4(+) T cell and MBC populations from a single clinical sample. This highly reproducible protocol has been successfully applied to multiple infectious disease settings to identify correlates of susceptibility or protection from infection. For complete details on the use and execution of this protocol, please refer to Ioannidis et al. (2021).
- Publisher
- Elsevier
- Keywords
- Cell Biology; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology; Systems biology
- Research Division(s)
- Infectious Diseases And Immune Defence
- PubMed ID
- 35378884
- Publisher's Version
- https://doi.org/10.1016/j.xpro.2022.101269
- Open Access at Publisher's Site
https://doi.org/10.1016/j.xpro.2022.101269- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2022-04-08 10:53:26
Last Modified: 2022-04-08 11:17:07