RhopH2 and RhopH3 export enables assembly of the RhopH complex on P. falciparum-infected erythrocyte membranes
- Author(s)
- Pasternak, M; Verhoef, JMJ; Wong, W; Triglia, T; Mlodzianoski, MJ; Geoghegan, N; Evelyn, C; Wardak, AZ; Rogers, K; Cowman, AF;
- Details
- Publication Year 2022-04-07,Volume 5,Issue #1,Page 333
- Journal Title
- Communications Biology
- Abstract
- RhopH complexes consists of Clag3, RhopH2 and RhopH3 and are essential for growth of Plasmodium falciparum inside infected erythrocytes. Proteins are released from rhoptry organelles during merozoite invasion and trafficked to the surface of infected erythrocytes and enable uptake of nutrients. RhopH3, unlike other RhopH proteins, is required for parasite invasion, suggesting some cellular processes RhopH proteins function as single players rather than a complex. We show the RhopH complex has not formed during merozoite invasion. Clag3 is directly released into the host cell cytoplasm, whilst RhopH2 and RhopH3 are released into the nascent parasitophorous vacuole. Export of RhopH2 and RhopH3 from the parasitophorous vacuole into the infected erythrocyte cytoplasm enables assembly of Clag3/RhopH2/RhopH3 complexes and incorporation into the host cell membrane concomitant with activation of nutrient uptake. This suggests compartmentalisation prevents premature channel assembly before intact complex is assembled at the host cell membrane.
- Publisher
- NPG
- Research Division(s)
- Structural Biology; Infectious Diseases And Immune Defence; Advanced Technology And Biology
- PubMed ID
- 35393572
- Publisher's Version
- https://doi.org/10.1038/s42003-022-03290-3
- Open Access at Publisher's Site
https://doi.org/10.1038/s42003-022-03290-3- NHMRC Grants
- NHMRC/1057960, NHMRC/1049811, NHMRC/1010326, NHMRC/637406,
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- Refer to copyright notice on published article.
Creation Date: 2022-04-11 11:15:10
Last Modified: 2022-07-13 02:50:09