Deficiency in coatomer complex I causes aberrant activation of STING signalling
Details
Publication Year 2022-04-28,Volume 13,Issue #1,Page 2321
Journal Title
Nature Communications
Abstract
Coatomer complex I (COPI) mediates retrograde vesicular trafficking from Golgi to the endoplasmic reticulum (ER) and within Golgi compartments. Deficiency in subunit alpha causes COPA syndrome and is associated with type I IFN signalling, although the upstream innate immune sensor involved was unknown. Using in vitro models we find aberrant activation of the STING pathway due to deficient retrograde but probably not intra-Golgi transport. Further we find the upstream cytosolic DNA sensor cGAS as essentially required to drive type I IFN signalling. Genetic deletion of COPI subunits COPG1 or COPD similarly induces type I IFN activation in vitro, which suggests that inflammatory diseases associated with mutations in other COPI subunit genes may exist. Finally, we demonstrate that inflammation in COPA syndrome patient peripheral blood mononuclear cells and COPI-deficient cell lines is ameliorated by treatment with the small molecule STING inhibitor H-151, suggesting targeted inhibition of the cGAS/STING pathway as a promising therapeutic approach.
Publisher
NPG
WEHI Research Division(s)
Inflammation
PubMed ID
35484149
Open Access at Publisher's Site
https://doi.org/0.1038/s41467-022-29946-6
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2022-05-03 09:18:43
Last Modified: 2022-05-03 09:40:39
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