Low-viscosity matrix suspension culture for human colorectal epithelial organoids and tumoroids
Details
Publication Year 2022-04-20,Volume 12,Issue #8,Page e4394
Journal Title
Bio-protocol
Abstract
Three-dimensional culture of human normal colorectal epithelium and cancer tissue as organoids and tumoroids has transformed the study of diseases of the large intestine. A widely used strategy for generating patient-derived colorectal organoids and tumoroids involves embedding cells in domes of extracellular matrix (ECM). Despite its success, dome culture is not ideal for scalable expansion, experimentation, and high-throughput screening applications. Our group has developed a protocol for growing patient-derived colorectal organoids and tumoroids in low-viscosity matrix (LVM) suspension culture. Instead of embedding colonic crypts or tumor fragments in solid ECM, these are grown suspended in medium containing only a low percentage of ECM. Compared with dome cultures, LVM suspension culture reduces the labor and cost of establishing and passaging organoids and tumoroids, enables rapid expansion, and is readily adaptable for high-throughput screening. Graphic abstract: Generation of organoids and tumoroids from human large intestine using LVM suspension culture (Created with BioRender.com).
Publisher
BioProtocol
Keywords
Colorectum; Low-viscosity matrix; Patient-derived organoids; Suspension culture; Tumoroids
Research Division(s)
Personalised Oncology
PubMed ID
35800090
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2022-07-11 12:21:28
Last Modified: 2022-07-11 12:38:42
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