BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation
- Author(s)
- Keniry, A; Jansz, N; Gearing, LJ; Wanigasuriya, I; Chen, J; Nefzger, CM; Hickey, PF; Gouil, Q; Liu, J; Breslin, KA; Iminitoff, M; Beck, T; Tapia Del Fierro, A; Whitehead, L; Jarratt, A; Kinkel, SA; Taberlay, PC; Willson, T; Pakusch, M; Ritchie, ME; Hilton, DJ; Polo, JM; Blewitt, ME;
- Details
- Publication Year 2022-03-29,Volume 13,Issue #1,Page 1658
- Journal Title
- Nature Communications
- Abstract
- The process of epigenetic silencing, while fundamentally important, is not yet completely understood. Here we report a replenishable female mouse embryonic stem cell (mESC) system, Xmas, that allows rapid assessment of X chromosome inactivation (XCI), the epigenetic silencing mechanism of one of the two X chromosomes that enables dosage compensation in female mammals. Through a targeted genetic screen in differentiating Xmas mESCs, we reveal that the BAF complex is required to create nucleosome-depleted regions at promoters on the inactive X chromosome during the earliest stages of establishment of XCI. Without this action gene silencing fails. Xmas mESCs provide a tractable model for screen-based approaches that enable the discovery of unknown facets of the female-specific process of XCI and epigenetic silencing more broadly.
- Publisher
- NPG
- Research Division(s)
- Advanced Technology And Biology; Blood Cells And Blood Cancer; Epigenetics And Development
- PubMed ID
- 35351876
- Publisher's Version
- https://doi.org/10.1038/s41467-022-29333-1
- Open Access at Publisher's Site
https://doi.org/10.1038/s41467-022-29333-1- NHMRC Grants
- NHMRC/1059624, NHMRC/1194345, NHMRC/1140976,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2022-07-13 02:56:02
Last Modified: 2022-07-13 02:57:09