Enhanced nitric oxide production by macrophages treated with a cell-penetrating peptide conjugate
- Author(s)
- Rahman, A; Matthews, MA; Nowell, CJ; Chalmers, DK; Thompson, PE; Nicholson, SE; Barlow, N; Norton, RS;
- Journal Title
- Bioorganic Chemistry
- Abstract
- The SPRY domain-containing SOCS box protein-2 (SPSB2) plays a critical role in the degradation of inducible nitric oxide synthase (iNOS) in macrophages. In this study, we have conjugated a peptide inhibitor of the iNOS-SPSB2 interaction with a cell-penetrating peptide (CPP) for delivery into macrophages, and confirmed its binding to SPSB2. We have assessed the uptake of a fluorophore-tagged analogue by RAW 264.7 and immortalised bone marrow derived macrophage (iBMDM) cell lines, and shown that the CPP-peptide conjugate enhanced NO production. The findings of this study will be useful in further refinement of CPP-peptide conjugates as leads in the development of new antibiotics that target the host innate immune response.
- Publisher
- Elsevier
- Keywords
- Cell-penetrating peptide; Macrophage; Pathogen killing; Spsb; iNOS
- Research Division(s)
- Inflammation
- PubMed ID
- 35366581
- Publisher's Version
- https://doi.org/10.1016/j.bioorg.2022.105763
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2022-07-15 08:45:04
Last Modified: 2022-07-15 09:06:21