Nanobodies identify an activated state of the TRIB2 pseudokinase
Journal Title
Structure
Abstract
Tribbles proteins (TRIB1-3) are pseudokinases that recruit substrates to the COP1 ubiquitin ligase. TRIB2 was the first Tribbles ortholog to be implicated as a myeloid leukemia oncogene, because it recruits the C/EBPα transcription factor for ubiquitination by COP1. Here we report identification of nanobodies that bind the TRIB2 pseudokinase domain with low nanomolar affinity. A crystal structure of the TRIB2-Nb4.103 complex identified the nanobody to bind the N-terminal lobe of TRIB2, enabling specific recognition of TRIB2 in an activated conformation that is similar to the C/EBPα-bound state of TRIB1. Characterization in solution revealed that Nb4.103 can stabilize a TRIB2 pseudokinase domain dimer in a face-to-face manner. Conversely, a distinct nanobody (Nb4.101) binds through a similar epitope but does not readily promote dimerization. In combination, this study identifies features of TRIB2 that could be exploited for the development of inhibitors and nanobody tools for future investigation of TRIB2 function.
Publisher
Elsevier
Keywords
Trib2; Tribbles; nanobody; pseudokinase
WEHI Research Division(s)
Inflammation
PubMed ID
36108635
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2022-09-19 02:39:11
Last Modified: 2022-09-23 09:51:01
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