Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF)
- Journal Title
- Methods in Molecular Biology
- Abstract
- Mass cytometry time-of-flight (CyTOF) is a technology for the study of complex biological processes at the single-cell level. The technology enables measurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological processes in a cell. Here, we describe this technology's use to profile changes in the intrinsic apoptotic (cell death) protein machinery at a single-cell level. We provide a comprehensive overview of a tailor-made set of cell survival/death antibodies, ideal staining conditions, and high-dimensional data analysis.
- Publisher
- Springer
- Keywords
- Apoptosis; BCL-2 family; Cell death; Mass cytometry time-of-flight (CyTOF); Single cell
- Research Division(s)
- Immunology
- PubMed ID
- 36087261
- Publisher's Version
- https://doi.org/10.1007/978-1-0716-2553-8_8
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2022-09-19 02:39:16
Last Modified: 2022-09-23 09:58:45