A specialized tyrosine-based endocytosis signal in MR1 controls antigen presentation to MAIT cells
- Lim, HJ; Wubben, JM; Garcia, CP; Cruz-Gomez, S; Deng, J; Mak, JYW; Hachani, A; Anderson, RJ; Painter, GF; Goyette, J; Amarasinghe, SL; Ritchie, ME; Roquilly, A; Fairlie, DP; Gaus, K; Rossjohn, J; Villadangos, JA; McWilliam, HEG;
- Publication Year 2022-12-05,Volume 221,Issue #12,Page e202110125
- Journal Title
- Journal of Cell Biology
- MR1 is a highly conserved microbial immune-detection system in mammals. It captures vitamin B-related metabolite antigens from diverse microbes and presents them at the cell surface to stimulate MR1-restricted lymphocytes including mucosal-associated invariant T (MAIT) cells. MR1 presentation and MAIT cell recognition mediate homeostasis through host defense and tissue repair. The cellular mechanisms regulating MR1 cell surface expression are critical to its function and MAIT cell recognition, yet they are poorly defined. Here, we report that human MR1 is equipped with a tyrosine-based motif in its cytoplasmic domain that mediates low affinity binding with the endocytic adaptor protein 2 (AP2) complex. This interaction controls the kinetics of MR1 internalization from the cell surface and minimizes recycling. We propose MR1 uses AP2 endocytosis to define the duration of antigen presentation to MAIT cells and the detection of a microbial metabolic signature by the immune system.
- Rockefeller University Press
- WEHI Research Division(s)
- Epigenetics And Development
- PubMed ID
- Publisher's Version
- Refer to copyright notice on published article.
Creation Date: 2022-09-23 10:02:54Last Modified: 2022-09-23 10:15:22