Arginine-rich C9ORF72 ALS proteins stall ribosomes in a manner distinct from a canonical ribosome-associated quality control substrate
- Author(s)
- Kriachkov, V; Ormsby, AR; Kusnadi, EP; McWilliam, HEG; Mintern, JD; Amarasinghe, SL; Ritchie, ME; Furic, L; Hatters, DM;
- Journal Title
- Journal of Biological Chemistry
- Publication Type
- epub ahead of print
- Abstract
- Hexanucleotide expansion mutations in C9ORF72 are a frequent cause of amyotrophic lateral sclerosis. We previously reported that long arginine-rich dipeptide repeats (DPR), mimicking abnormal proteins expressed from the hexanucleotide expansion, caused translation stalling when expressed in cell culture models. Whether this stalling provides a mechanism of pathogenicity remains to be determined. Here we explored the molecular features of DPR-induced stalling and examined whether known mechanisms such as ribosome quality control (RQC) regulate translation elongation on sequences that encode arginine-rich DPRs. We demonstrate that arginine-rich DPRs lead to stalling in a length-dependent manner, with lengths longer than 40 repeats invoking severe translation arrest. Mutational screening of 40×Gly-Xxx DPRs shows that stalling is most pronounced when Xxx is a charged amino acid (Arg, Lys, Glu, or Asp). Through a genome-wide knockout screen, we find that genes regulating stalling on polyadenosine mRNA coding for poly-Lys, a canonical RQC substrate, act differently in the case of arginine-rich DPRs. Indeed, these findings point to a limited scope for natural regulatory responses to resolve the arginine-rich DPR stalls, even though the stalls may be sensed, as evidenced by an upregulation of RQC gene expression. These findings therefore implicate arginine-rich DPR-mediated stalled ribosomes as a source of stress and toxicity and may be a crucial component in pathomechanisms.
- Publisher
- Elsevier
- Keywords
- C9orf72 als; RNA-protein interaction; arginine-rich dipeptide repeats; neurodegenerative disease; protein misfolding; ribosome function; ribosome stalling; translation
- Research Division(s)
- Epigenetics And Development
- PubMed ID
- 36481270/
- Publisher's Version
- https://doi.org/10.1016/j.jbc.2022.102774
- Open Access at Publisher's Site
https://doi.org/ 10.1016/j.jbc.2022.102774- Terms of Use/Rights Notice
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Creation Date: 2022-12-13 03:11:33
Last Modified: 2022-12-14 01:19:25