A method for stabilising the XX karyotype in female mESC cultures
- Author(s)
- Keniry, A; Jansz, N; Hickey, PF; Breslin, KA; Iminitoff, M; Beck, T; Gouil, Q; Ritchie, ME; Blewitt, ME;
- Journal Title
- Development
- Abstract
- Female mouse embryonic stem cells (mESCs) present differently from male mESCs in several fundamental ways; however, complications with their in vitro culture have resulted in an under-representation of female mESCs in the literature. Recent studies show that the second X chromosome in female, and more specifically the transcriptional activity from both of these chromosomes due to absent X chromosome inactivation, sets female and male mESCs apart. To avoid this undesirable state, female mESCs in culture preferentially adopt an XO karyotype, with this adaption leading to loss of their unique properties in favour of a state that is near indistinguishable from male mESCs. If female pluripotency is to be studied effectively in this system, it is crucial that high-quality cultures of XX mESCs are available. Here, we report a method for better maintaining XX female mESCs in culture that also stabilises the male karyotype and makes study of female-specific pluripotency more feasible.
- Publisher
- COB
- Keywords
- Male; Animals; Female; Mice; Mouse Embryonic Stem Cells; Cell Differentiation/physiology; X Chromosome Inactivation/genetics; Karyotype; Cell culture; Embryonic stem cells; X chromosome inactivation
- Research Division(s)
- Epigenetics And Development; Advanced Technology And Biology
- PubMed ID
- 36355065
- Publisher's Version
- https://doi.org/10.1242/dev.200845
- Open Access at Publisher's Site
https://doi.org/10.1242/dev.200845- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2022-12-13 03:12:52
Last Modified: 2022-12-13 03:52:57