The RNA-binding protein SRSF3 has an essential role in megakaryocyte maturation and platelet production
Details
Publication Year 2022-03-03,Volume 139,Issue #9,Page 1359-1373
Journal Title
Blood
Abstract
RNA processing is increasingly recognized as a critical control point in the regulation of different hematopoietic lineages including megakaryocytes responsible for the production of platelets. Platelets are anucleate cytoplasts that contain a rich repertoire of RNAs encoding proteins with essential platelet functions derived from the parent megakaryocyte. It is largely unknown how RNA binding proteins contribute to the development and functions of megakaryocytes and platelets. We show that serine-arginine-rich splicing factor 3 (SRSF3) is essential for megakaryocyte maturation and generation of functional platelets. Megakaryocyte-specific deletion of Srsf3 in mice led to macrothrombocytopenia characterized by megakaryocyte maturation arrest, dramatically reduced platelet counts, and abnormally large functionally compromised platelets. SRSF3 deficient megakaryocytes failed to reprogram their transcriptome during maturation and to load platelets with RNAs required for normal platelet function. SRSF3 depletion led to nuclear accumulation of megakaryocyte mRNAs, demonstrating that SRSF3 deploys similar RNA regulatory mechanisms in megakaryocytes as in other cell types. Our study further suggests that SRSF3 plays a role in sorting cytoplasmic megakaryocyte RNAs into platelets and demonstrates how SRSF3-mediated RNA processing forms a central part of megakaryocyte gene regulation. Understanding SRSF3 functions in megakaryocytes and platelets provides key insights into normal thrombopoiesis and platelet pathologies as SRSF3 RNA targets in megakaryocytes are associated with platelet diseases.
Publisher
Elsevier
Keywords
Animals; Blood Platelets/*metabolism; Megakaryocytes/*metabolism; Mice; Mice, Knockout; RNA, Messenger/genetics/metabolism; Serine-Arginine Splicing Factors/genetics/metabolism; *Thrombocytopenia/genetics/metabolism; Thrombopoiesis/*genetics
Research Division(s)
Blood Cells And Blood Cancer
PubMed ID
34852174
Open Access at Publisher's Site
https://doi.org/10.1182/blood.2021013826
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2023-02-24 09:21:05
Last Modified: 2023-02-24 09:27:05
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