S100 family proteins are linked to organoid morphology and EMT in pancreatic cancer

Low, Ronnie Ren Jie; Fung, Ka Yee; Gao, Hugh; Preaudet, Adele; Dagley, Laura F; Yousef, Jumana; Lee, Belinda; Emery-Corbin, Samantha J; Nguyen, Paul M; Larsen, Rune H; Kershaw, Nadia J; Burgess, Antony W; Gibbs, Peter; Hollande, Fr&#233;d&#233;ric; Griffin, Michael DW; Grimmond, Sean M; Putoczki, Tracy L

Author(s): Low, Ronnie Ren Jie; Fung, Ka Yee; Gao, Hugh; Preaudet, Adele; Dagley, Laura F; Yousef, Jumana; Lee, Belinda; Emery-Corbin, Samantha J; Nguyen, Paul M; Larsen, Rune H; Kershaw, Nadia J; Burgess, Antony W; Gibbs, Peter; Hollande, Frédéric; Griffin, Michael DW; Grimmond, Sean M; Putoczki, Tracy L;
Details: Publication Year 2023-02-24,Volume 30,Issue #5,Page 1155-1165
Journal Title: Cell Death & Differ
Abstract: Epithelial-mesenchymal transition (EMT) is a continuum that includes epithelial, partial EMT, and mesenchymal states, each of which is associated with cancer progression, invasive capabilities, and ultimately, metastasis. We used a lineage-traced sporadic model of pancreatic cancer to generate a murine organoid biobank from primary and secondary tumors, including sublines that underwent partial EMT and complete EMT. Using an unbiased proteomics approach, we found that organoid morphology predicts the EMT state, and the solid organoids are associated with a partial EMT signature. We also observed that exogenous TGFβ1 induces solid organoid morphology that is associated with changes in the S100 family, complete EMT, and the formation of high-grade tumors. S100A4 may be a useful biomarker for predicting EMT state, disease progression, and outcome in patients with pancreatic cancer.
Publisher: NPG
Keywords: Humans; Animals; Mice; S100 Proteins/genetics/metabolism; Epithelial-Mesenchymal Transition; Pancreatic Neoplasms/pathology; Cell Line, Tumor
Research Division(s): Personalised Oncology; Structural Biology; Advanced Technology And Biology; Advanced Technology and Biology; Structural Biology
PubMed ID: 36828915
Publisher's Version: https://doi.org/10.1038/s41418-023-01126-z
Open Access at Publisher's Site: https://doi.org/10.1038/s41418-023-01126-z
Terms of Use/Rights Notice: Refer to copyright notice on published article.
Documents: s41418-023-01126-z.pdf - (3.85MB, application/pdf)

Creation Date: 2023-04-13 11:29:15

Last Modified: 2023-06-13 01:17:48