Distinct subpopulations of DN1 thymocytes exhibit preferential γδ T lineage potential
- Author(s)
- Oh, S; Liu, X; Tomei, S; Luo, M; Skinner, JP; Berzins, SP; Naik, SH; Gray, DHD; Chong, MMW;
- Journal Title
- Frontiers in Immunology
- Abstract
- The αβ and γδ T cell lineages both differentiate in the thymus from common uncommitted progenitors. The earliest stage of T cell development is known as CD4(-)CD8(-) double negative 1 (DN1), which has previously been shown to be a heterogenous mixture of cells. Of these, only the CD117(+) fraction has been proposed to be true T cell progenitors that progress to the DN2 and DN3 thymocyte stages, at which point the development of the αβ and γδ T cell lineages diverge. However, recently, it has been shown that at least some γδ T cells may be derived from a subset of CD117(-) DN thymocytes. Along with other ambiguities, this suggests that T cell development may not be as straightforward as previously thought. To better understand early T cell development, particularly the heterogeneity of DN1 thymocytes, we performed a single cell RNA sequence (scRNAseq) of mouse DN and γδ thymocytes and show that the various DN stages indeed comprise a transcriptionally diverse subpopulations of cells. We also show that multiple subpopulations of DN1 thymocytes exhibit preferential development towards the γδ lineage. Furthermore, specific γδ-primed DN1 subpopulations preferentially develop into IL-17 or IFNγ-producing γδ T cells. We show that DN1 subpopulations that only give rise to IL-17-producing γδ T cells already express many of the transcription factors associated with type 17 immune cell responses, while the DN1 subpopulations that can give rise to IFNγ-producing γδ T cell already express transcription factors associated with type 1 immune cell responses.
- Publisher
- Frontiers Media
- Keywords
- Mice; Animals; *Thymocytes; *Interleukin-17/metabolism; Thymus Gland; Cell Differentiation; Transcription Factors/metabolism; T cell development; gamma delta (γδ) T cells; lineage decision; scRNAseq; thymocyte
- Research Division(s)
- Immunology
- PubMed ID
- 37077921
- Publisher's Version
- https://doi.org/10.3389/fimmu.2023.1106652
- Open Access at Publisher's Site
https://doi.org/10.3389/fimmu.2023.1106652- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-05-01 02:16:46
Last Modified: 2023-05-03 10:00:56