Consensus Report on Markers to Distinguish Procoagulant Platelets from Apoptotic Platelets: Communication from the SSC of the ISTH

Josefsson, EC; Ramstr&#246;m, S; Thaler, J; Lordkipanidz&#233;, M

Author(s): Josefsson, EC; Ramström, S; Thaler, J; Lordkipanidzé, M;
Details: Publication Year 2023-05-10,Volume 21,Issue #8,Page 2291-2299
Journal Title: Journal of Thrombosis and Haemostasis
Abstract: BACKGROUND: Procoagulant platelets are a subpopulation of highly activated platelets that promote coagulation through surface-exposed, negatively charged phospholipids, especially phosphatidylserine (PS). Procoagulant platelets are important for clot stabilization during haemostasis and an increased number of these platelets is associated with thrombotic risk. There is a need for harmonisation in this area since many of the markers and methods used to assess procoagulant platelets are not specific when used in isolation but are also associated with platelet apoptosis. OBJECTIVE: We initiated this project to identify a minimum set of markers and/or methods that can detect and distinguish procoagulant platelets from apoptotic platelets. METHODS AND RESULTS: The study design involved a primary panel with twenty-seven international experts participating in an online survey and moderated virtual focus group meetings. Primary and secondary panel members were then invited to provide input on themes and statements generated from the focus groups. This led to a recommendation to use flow cytometry and a combination of the following three surface markers to differentiate procoagulant from apoptotic platelets: P-selectin (CD62P), PS (recognized by annexin V), and a platelet-specific receptor GPIX (CD42a) or αIIb integrin (CD41, GPIIb). CONCLUSION: Procoagulant platelets are expected to be positive for all three markers, while apoptotic platelets will be positive for annexin V and the platelet specific surface receptor(s) but negative for P-selectin.
Publisher: Elsevier
Keywords: Annexin V; Apoptosis; Flow cytometry; P-selectin; Platelet activation
Research Division(s): Cancer Biology And Stem Cells
PubMed ID: 37172731
Publisher's Version: https://doi.org/10.1016/j.jtha.2023.05.001
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2023-06-26 09:53:27

Last Modified: 2023-12-13 10:19:50