A Streamlined Method for Detecting Inflammasome-Induced ASC Oligomerization Using Chemical Crosslinking
- Author(s)
- Hughes, SA; Vince, JE;
- Details
- Publication Year 2023,Volume 2691,Issue #vol 2691,Page 155-164
- Journal Title
- Methods in Molecular Biology
- Abstract
- The apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) functions as the integral adaptor protein between inflammasome sensors such as NOD-like receptor protein 3 (NLRP3) and the inflammatory caspase, caspase-1. Inflammasome sensor triggering allows recruitment of ASC and the formation of long amyloid-like ASC oligomers that enable binding and proximity-induced activation of caspase-1. The detection of ASC oligomerization thus constitutes a highly specific and direct test for inflammasome complex formation and activation. Here, we describe a simplified and streamlined method for the detection of ASC oligomers via Western blotting, using the chemical crosslinking reagent disuccinimidyl suberate.
- Publisher
- Humana
- Keywords
- Asc; Caspase-1; Cell death; Inflammasome; Inflammation; Innate immunity; Interleukin-1; Nlrp3; Pyroptosis
- Research Division(s)
- Inflammation
- PubMed ID
- 37355544
- Publisher's Version
- https://doi.org/10.1007/978-1-0716-3331-1_12
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-06-30 02:16:08
Last Modified: 2023-12-13 10:19:50