On Target: An Intrapulmonary Transplantation Method for Modelling Lung Tumor Development in its Native Microenvironment
- Author(s)
- McDonald, JA; Scott, L; Van Zuylekom, J; Holloway, S; Blyth, BJ; Sutherland, KD;
- Journal Title
- Methods in Molecular Biology
- Publication Type
- Book Chapter
- Abstract
- The development of in vivo lung cancer models that faithfully mimic the human disease is a crucial research tool for understanding the molecular mechanisms driving tumorigenesis. Subcutaneous transplantation assays are commonly employed, likely due to their amenability to easily monitor tumor growth and the simplistic nature of the technique to deliver tumor cells. Importantly however, subcutaneous tumors grow in a microenvironment that differs from that resident within the lung. To circumvent this limitation, here we describe the development of an intrapulmonary (iPUL) orthotopic transplantation method that enables the delivery of lung cancer cells, with precision, to the left lung lobe of recipient mice. Critically, this allows for the growth of lung cancer cells within their native microenvironment. The coupling of iPUL transplantation with position emission tomography (PET) imaging permits the serial detection of tumors in vivo and serves as a powerful tool to trace lung tumor growth and dissemination over time in mouse disease models.
- Publisher
- Humana
- Keywords
- Intrapulmonary injection; Lung cancer; Orthotopic transplantation; PET/CT imaging; Preclinical imaging; Tumor microenvironment
- Research Division(s)
- Cancer Biology And Stem Cells
- PubMed ID
- 37355535
- Publisher's Version
- https://doi.org/10.1007/978-1-0716-3331-1_3
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-06-30 02:16:12
Last Modified: 2023-06-30 02:53:33