Flow Cytometry Identification of Cell Compartments in the Murine Brain
- Author(s)
- Moffet, JJD; Moore, Z; Oliver, SJ; Towers, T; Jenkins, MR; Freytag, S; Whittle, JR; Best, SA;
- Journal Title
- Methods in Molecular Biology
- Abstract
- Glioma can be modelled in the murine brain through the induction of genetically engineered mouse models or intracranial transplantation. Gliomas (oligodendroglioma and astrocytoma) are thought to arise from neuronal and glial progenitor populations in the brain and are poorly infiltrated by immune cells. An improved understanding of oligodendrocytes, astrocytes, and the immune environment throughout tumor development will enhance the analysis and development of brain cancer models. Here, we describe the isolation and analysis of murine brain cell types using a combination of flow cytometry and quantitative RT-PCR strategies to analyze these individual cell populations in vivo.
- Publisher
- Humana
- Keywords
- Brain; Flow cytometry; Glial cells; Quantitative RT-PCR; Tissue dissociation
- Research Division(s)
- Personalised Oncology; Immunology
- PubMed ID
- 37355546
- Publisher's Version
- https://doi.org/10.1007/978-1-0716-3331-1_14
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-06-30 02:16:14
Last Modified: 2023-06-30 02:56:44