Parallel use of human stem cell lung and heart models provide insights for SARS-CoV-2 treatment

Rudraraju, R; Gartner, MJ; Neil, JA; Stout, ES; Chen, J; Needham, EJ; See, M; Mackenzie-Kludas, C; Yang Lee, LY; Wang, M; Pointer, H; Karavendzas, K; Abu-Bonsrah, D; Drew, D; Yang Sun, YB; Tan, JP; Sun, G; Salavaty, A; Charitakis, N; Nim, HT; Currie, PD; Tham, WH; Porrello, E; Polo, JM; Humphrey, SJ; Ramialison, M; Elliott, DA; Subbarao, K

Author(s): Rudraraju, R; Gartner, MJ; Neil, JA; Stout, ES; Chen, J; Needham, EJ; See, M; Mackenzie-Kludas, C; Yang Lee, LY; Wang, M; Pointer, H; Karavendzas, K; Abu-Bonsrah, D; Drew, D; Yang Sun, YB; Tan, JP; Sun, G; Salavaty, A; Charitakis, N; Nim, HT; Currie, PD; Tham, WH; Porrello, E; Polo, JM; Humphrey, SJ; Ramialison, M; Elliott, DA; Subbarao, K;
Details: Publication Year 2023-06-13,Volume 18,Issue #6,Page 1308-1324
Journal Title: Stem Cell Reports
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily infects the respiratory tract, but pulmonary and cardiac complications occur in severe coronavirus disease 2019 (COVID-19). To elucidate molecular mechanisms in the lung and heart, we conducted paired experiments in human stem cell-derived lung alveolar type II (AT2) epithelial cell and cardiac cultures infected with SARS-CoV-2. With CRISPR-Cas9-mediated knockout of ACE2, we demonstrated that angiotensin-converting enzyme 2 (ACE2) was essential for SARS-CoV-2 infection of both cell types but that further processing in lung cells required TMPRSS2, while cardiac cells required the endosomal pathway. Host responses were significantly different; transcriptome profiling and phosphoproteomics responses depended strongly on the cell type. We identified several antiviral compounds with distinct antiviral and toxicity profiles in lung AT2 and cardiac cells, highlighting the importance of using several relevant cell types for evaluation of antiviral drugs. Our data provide new insights into rational drug combinations for effective treatment of a virus that affects multiple organ systems.
Publisher: Elsevier
Keywords: Covid-19; SARS-CoV-2; antiviral drugs; human stem cell-derived lung and heart models
Research Division(s): Infectious Diseases And Immune Defence
PubMed ID: 37315523
Publisher's Version: https://doi.org/10.1016/j.stemcr.2023.05.007
Open Access at Publisher's Site: https://doi.org/10.1016/j.stemcr.2023.05.007
Terms of Use/Rights Notice: Refer to copyright notice on published article.
Documents: PIIS2213671123001856.pdf - (5.06MB, application/pdf)

Creation Date: 2023-06-30 02:16:19

Last Modified: 2023-06-30 03:03:32