Serological assays to measure dimeric IgA antibodies in SARS-CoV-2 infections

Wei, Z; Angrisano, F; Eriksson, EM; Mazhari, R; Van, H; Zheng, S; Center, RJ; McMahon, J; Lau, J; Kiernan-Walker, N; Ruybal-Pes&#225;ntez, S; Mueller, I; Robinson, LJ; Anderson, DA; Drummer, HE

Author(s): Wei, Z; Angrisano, F; Eriksson, EM; Mazhari, R; Van, H; Zheng, S; Center, RJ; McMahon, J; Lau, J; Kiernan-Walker, N; Ruybal-Pesántez, S; Mueller, I; Robinson, LJ; Anderson, DA; Drummer, HE;
Details: Publication Year 2023-08-18,Volume 101,Issue #9,Page 857-866
Journal Title: Immunology and Cell Biology
Publication Type: epub ahead of print
Abstract: Current serological tests cannot differentiate between total immunoglobulin A (IgA) and dimeric IgA (dIgA) associated with mucosal immunity. Here, we describe two new assays, dIgA-ELISA and dIgA-multiplex bead assay (MBA), that utilize the preferential binding of dIgA to a chimeric form of secretory component, allowing the differentiation between dIgA and monomeric IgA. dIgA responses elicited through severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection were measured in (i) a longitudinal panel, consisting of 74 samples (n = 20 individuals) from hospitalized cases of coronavirus disease 2019 (COVID-19); (ii) a longitudinal panel, consisting of 96 samples (n = 10 individuals) from individuals with mild COVID-19; (iii) a cross-sectional panel with PCR-confirmed SARS-CoV-2 infection with mild COVID-19 (n = 199) and (iv) pre-COVID-19 samples (n = 200). The dIgA-ELISA and dIgA-MBA demonstrated a specificity for dIgA of 99% and 98.5%, respectively. Analysis of dIgA responses in the longitudinal panels revealed that 70% (ELISA) and 50% (MBA) of patients elicited a dIgA response by day 20 after PCR diagnosis with a SARS-CoV-2 infection. Individuals with mild COVID-19 displayed increased levels of dIgA within the first 3 weeks after diagnosis but responses appeared to be short lived, compared with sustained IgA levels. However, in samples from hospitalized patients with COVID-19 we observed high and sustained levels of dIgA, up to 245 days after PCR diagnosis. Our results suggest that severe COVID-19 infections are associated with sustained levels of plasma dIgA compared with mild cases.
Publisher: Wiley
Keywords: Covid-19; dIgA; mucosal immunity; serological assay
Research Division(s): Population Health And Immunity
PubMed ID: 37593973
Publisher's Version: https://doi.org/10.1111/imcb.12682
Open Access at Publisher's Site: https://doi.org/10.1111/imcb.12682
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2023-08-28 09:37:21

Last Modified: 2023-11-20 03:24:10