Molecular basis of sulfolactate synthesis by sulfolactaldehyde dehydrogenase from Rhizobium leguminosarum
- Author(s)
- Li, J; Sharma, M; Meek, R; Alhifthi, A; Armstrong, Z; Soler, NM; Lee, M; Goddard-Borger, ED; Blaza, JN; Davies, GJ; Williams, SJ;
- Details
- Publication Year 2023-10-25,Volume 14,Issue #41,Page 11429-11440
- Journal Title
- Chemical Science
- Abstract
- Sulfolactate (SL) is a short-chain organosulfonate that is an important reservoir of sulfur in the biosphere. SL is produced by oxidation of sulfolactaldehyde (SLA), which in turn derives from sulfoglycolysis of the sulfosugar sulfoquinovose, or through oxidation of 2,3-dihydroxypropanesulfonate. Oxidation of SLA is catalyzed by SLA dehydrogenases belonging to the aldehyde dehydrogenase superfamily. We report that SLA dehydrogenase RlGabD from the sulfoglycolytic bacterium Rhizobium leguminsarum SRDI565 can use both NAD(+) and NADP(+) as cofactor to oxidize SLA, and indicatively operates through a rapid equilibrium ordered mechanism. We report the cryo-EM structure of RlGabD bound to NADH, revealing a tetrameric quaternary structure and supporting proposal of organosulfonate binding residues in the active site, and a catalytic mechanism. Sequence based homology searches identified SLA dehydrogenase homologs in a range of putative sulfoglycolytic gene clusters in bacteria predominantly from the phyla Actinobacteria, Firmicutes, and Proteobacteria. This work provides a structural and biochemical view of SLA dehydrogenases to complement our knowledge of SLA reductases, and provide detailed insights into a critical step in the organosulfur cycle.
- Publisher
- Royal Society of Chemistry
- Research Division(s)
- Chemical Biology
- PubMed ID
- 37886098
- Publisher's Version
- https://doi.org/10.1039/d3sc01594g
- Open Access at Publisher's Site
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Creation Date: 2023-11-15 05:00:10
Last Modified: 2023-11-15 05:29:55