Tumor immune evasion: insights from CRISPR screens and future directions
Details
Publication Year 2024-11-16,Volume 291,Issue #7,Page 1386-1399
Journal Title
FEBS Journal
Abstract
Despite the clinical success of cancer immunotherapies including immune checkpoint blockade (ICB) and adoptive cellular therapies (ACTs) across a variety of cancer types, many patients do not respond or ultimately relapse, however, the molecular underpinnings of this are not fully understood. Thus, a systems level understating of the routes to tumor immune evasion is required to inform the design of the next generation of immunotherapy approaches. CRISPR screening approaches have proved extremely powerful in identifying genes that promote tumor immune evasion or sensitize tumor cells to destruction by the immune system. These large-scale efforts have brought to light decades worth of fundamental immunology and have uncovered the key immune-evasion pathways subverted in cancers in an acquired manner in patients receiving immune-modulatory therapies. The comprehensive discovery of the main pathways involved in immune-evasion has spurred the development and application of novel immune-therapies to target this process. Although successful, conventional CRISPR-screening approaches are hampered by a number of limitations which obfuscate a complete understanding of the precise molecular regulation of immune-evasion in cancer. Here, we provide a perspective on screening approaches to interrogate tumor-lymphocyte interactions and their limitations, and discuss further development of technologies to improve such approaches and discovery capability.
Publisher
Wiley
Keywords
Crispr; immune evasion; lymphocytes; screening; technology
Research Division(s)
Ubiquitin Signalling; Advanced Technology And Biology; Ubiquitin Signalling
PubMed ID
37971319
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2023-11-20 03:32:51
Last Modified: 2024-06-28 03:14:57
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