Widespread Family of NAD(+)-Dependent Sulfoquinovosidases at the Gateway to Sulfoquinovose Catabolism
- Author(s)
- Kaur, A; Pickles, IB; Sharma, M; Madeido Soler, N; Scott, NE; Pidot, SJ; Goddard-Borger, ED; Davies, GJ; Williams, SJ;
- Journal Title
- Journal of the American Chemical Society
- Publication Type
- epub ahead of print
- Abstract
- The sulfosugar sulfoquinovose (SQ) is produced by photosynthetic plants, algae, and cyanobacteria on a scale of 10 billion tons per annum. Its degradation, which is essential to allow cycling of its constituent carbon and sulfur, involves specialized glycosidases termed sulfoquinovosidases (SQases), which release SQ from sulfolipid glycoconjugates, so SQ can enter catabolism pathways. However, many SQ catabolic gene clusters lack a gene encoding a classical SQase. Here, we report the discovery of a new family of SQases that use an atypical oxidoreductive mechanism involving NAD(+) as a catalytic cofactor. Three-dimensional X-ray structures of complexes with SQ and NAD(+) provide insight into the catalytic mechanism, which involves transient oxidation at C3. Bioinformatic survey reveals this new family of NAD(+)-dependent SQases occurs within sulfoglycolytic and sulfolytic gene clusters that lack classical SQases and is distributed widely including within Roseobacter clade bacteria, suggesting an important contribution to marine sulfur cycling.
- Publisher
- ACS
- Research Division(s)
- Chemical Biology
- PubMed ID
- 38100472
- Publisher's Version
- https://doi.org/10.1021/jacs.3c11126
- Open Access at Publisher's Site
https://doi.org/10.1021/jacs.3c11126- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-12-21 07:38:39
Last Modified: 2023-12-21 07:44:44