Whole-genome CRISPR screens to understand Apicomplexan-host interactions

Author(s): Hesping, E; Boddey, JA;
Details: Publication Year 2024-01-15,Volume 121,Issue #4,Page 717-726
Journal Title: Molecular Microbiology
Abstract: Apicomplexan parasites are aetiological agents of numerous diseases in humans and livestock. Functional genomics studies in these parasites enable the identification of biological mechanisms and protein functions that can be targeted for therapeutic intervention. Recent improvements in forward genetics and whole-genome screens utilising CRISPR/Cas technology have revolutionised the functional analysis of genes during Apicomplexan infection of host cells. Here, we highlight key discoveries from CRISPR/Cas9 screens in Apicomplexa or their infected host cells and discuss remaining challenges to maximise this technology that may help answer fundamental questions about parasite-host interactions.
Publisher: Wiley
Keywords: Apicomplexa; Crispr; Cas9; forward genetics; host; parasite
Research Division(s): Infectious Diseases And Immune Defence
PubMed ID: 38225194
Publisher's Version: https://doi.org/10.1111/mmi.15221
Open Access at Publisher's Site: https://doi.org/10.1111/mmi.15221
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2024-01-25 09:18:00

Last Modified: 2024-05-09 09:00:52