A Broad-Spectrum α-Glucosidase of Glycoside Hydrolase Family 13 from Marinovum sp., a Member of the Roseobacter Clade
- Author(s)
- Li, J; Mui, JW; da Silva, BM; Pires, DEV; Ascher, DB; Madiedo Soler, N; Goddard-Borger, ED; Williams, SJ;
- Journal Title
- Applied Biochemistry and Biotechnology
- Publication Type
- epub ahead of print
- Abstract
- Glycoside hydrolases (GHs) are a diverse group of enzymes that catalyze the hydrolysis of glycosidic bonds. The Carbohydrate-Active enZymes (CAZy) classification organizes GHs into families based on sequence data and function, with fewer than 1% of the predicted proteins characterized biochemically. Consideration of genomic context can provide clues to infer possible enzyme activities for proteins of unknown function. We used the MultiGeneBLAST tool to discover a gene cluster in Marinovum sp., a member of the marine Roseobacter clade, that encodes homologues of enzymes belonging to the sulfoquinovose monooxygenase pathway for sulfosugar catabolism. This cluster lacks a gene encoding a classical family GH31 sulfoquinovosidase candidate, but which instead includes an uncharacterized family GH13 protein (MsGH13) that we hypothesized could be a non-classical sulfoquinovosidase. Surprisingly, recombinant MsGH13 lacks sulfoquinovosidase activity and is a broad-spectrum α-glucosidase that is active on a diverse array of α-linked disaccharides, including maltose, sucrose, nigerose, trehalose, isomaltose, and kojibiose. Using AlphaFold, a 3D model for the MsGH13 enzyme was constructed that predicted its active site shared close similarity with an α-glucosidase from Halomonas sp. H11 of the same GH13 subfamily that shows narrower substrate specificity.
- Publisher
- Springer
- Keywords
- Bioinformatics; Carbohydrates; Enzymes; Glycosidase
- Research Division(s)
- Chemical Biology
- PubMed ID
- 38180643
- Publisher's Version
- https://doi.org/10.1007/s12010-023-04820-3
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-01-25 09:18:03
Last Modified: 2024-01-25 10:47:14