Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the phagophore

Rao, S; Skulsuppaisarn, M; Strong, LM; Ren, X; Lazarou, M; Hurley, JH; Hummer, G

Author(s): Rao, S; Skulsuppaisarn, M; Strong, LM; Ren, X; Lazarou, M; Hurley, JH; Hummer, G;
Details: Publication Year 2024-02-09,Volume 10,Issue #6,Page eadj8027
Journal Title: Scientific Advances
Abstract: The covalent attachment of ubiquitin-like LC3 proteins (microtubule-associated proteins 1A/1B light chain 3) prepares the autophagic membrane for cargo recruitment. We resolve key steps in LC3 lipidation by combining molecular dynamics simulations and experiments in vitro and in cellulo. We show how the E3-like ligaseautophagy-related 12 (ATG12)-ATG5-ATG16L1 in complex with the E2-like conjugase ATG3 docks LC3 onto the membrane in three steps by (i) the phosphatidylinositol 3-phosphate effector protein WD repeat domain phosphoinositide-interacting protein 2 (WIPI2), (ii) helix α2 of ATG16L1, and (iii) a membrane-interacting surface of ATG3. Phosphatidylethanolamine (PE) lipids concentrate in a region around the thioester bond between ATG3 and LC3, highlighting residues with a possible role in the catalytic transfer of LC3 to PE, including two conserved histidines. In a near-complete pathway from the initial membrane recruitment to the LC3 lipidation reaction, the three-step targeting of the ATG12-ATG5-ATG16L1 machinery establishes a high level of regulatory control.
Publisher: AAAS
Keywords: Autophagy-Related Proteins/genetics/metabolism; *Autophagosomes/metabolism; *Microtubule-Associated Proteins/metabolism; Phagocytosis; Autophagy
Research Division(s): Ubiquitin Signalling
PubMed ID: 38324698
Publisher's Version: https://doi.org/10.1126/sciadv.adj8027
Open Access at Publisher's Site: https://doi.org/10.1126/sciadv.adj8027
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2024-02-29 09:01:59

Last Modified: 2024-02-29 09:12:57