Patient-Derived Xenograft Models for Leukemias
- Author(s)
- Brown, FC; Carmichael, CL;
- Journal Title
- Methods in Molecular Biology
- Abstract
- Patient-derived xenograft (PDX) modeling is a valuable tool for the study of leukemia pathogenesis, progression, and therapy response. Engraftment of human leukemia cells occurs following injection into the tail vein (or retro-orbital vein) of preconditioned immunocompromised mice. Injected mice are maintained in a sterile and supportive housing environment until leukemia engraftment is observed, at which time studies such as drug treatments or leukemia sampling can occur. Here, we outline a method for generating PDXs from Acute Myeloid Leukemia (AML) patient samples using tail vein injection; however it can also be readily applied to T- and B- Acute Lymphoblastic Leukemia (ALL) samples.
- Publisher
- Springer Nature
- Keywords
- Animals; Humans; Mice; *Disease Models, Animal; Xenograft Model Antitumor Assays/methods; Leukemia, Myeloid, Acute/pathology; Heterografts; Leukemia/pathology; Bone marrow; Immunocompromised; Irradiation; Leukemia; Mouse model; Peripheral blood; Spleen; Tail vein injection; Xenograft
- Research Division(s)
- Blood Cells And Blood Cancer
- PubMed ID
- 38676794
- Publisher's Version
- https://doi.org/10.1007/978-1-0716-3858-3_4
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-05-08 10:06:32
Last Modified: 2024-05-08 10:19:03