Mycobacterium ulcerans challenge strain selection for a Buruli ulcer controlled human infection model

Muhi, S; Buultjens, AH; Porter, JL; Marshall, JL; Doerflinger, M; Pidot, SJ; O&#39;Brien, DP; Johnson, PDR; Lavender, CJ; Globan, M; Mccarthy, J; Osowicki, J; Stinear, TP

Author(s): Muhi, S; Buultjens, AH; Porter, JL; Marshall, JL; Doerflinger, M; Pidot, SJ; O'Brien, DP; Johnson, PDR; Lavender, CJ; Globan, M; Mccarthy, J; Osowicki, J; Stinear, TP;
Details: Publication Year 2024-05-03,Volume 18,Issue #5,Page e0011979
Journal Title: PLOS Neglected Tropical DIseases
Abstract: Critical scientific questions remain regarding infection with Mycobacterium ulcerans, the organism responsible for the neglected tropical disease, Buruli ulcer (BU). A controlled human infection model has the potential to accelerate our knowledge of the immunological correlates of disease, to test prophylactic interventions and novel therapeutics. Here we present microbiological evidence supporting M. ulcerans JKD8049 as a suitable human challenge strain. This non-genetically modified Australian isolate is susceptible to clinically relevant antibiotics, can be cultured in animal-free and surfactant-free media, can be enumerated for precise dosing, and has stable viability following cryopreservation. Infectious challenge of humans with JKD8049 is anticipated to imitate natural infection, as M. ulcerans JKD8049 is genetically stable following in vitro passage and produces the key virulence factor, mycolactone. Also reported are considerations for the manufacture, storage, and administration of M. ulcerans JKD8049 for controlled human infection.
Publisher: PLOS
Research Division(s): Infectious Diseases And Immune Defence
PubMed ID: 38701090
Publisher's Version: https://doi.org/10.1371/journal.pntd.0011979
Open Access at Publisher's Site: https://doi.org/ 10.1371/journal.pntd.0011979
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2024-05-08 10:06:37

Last Modified: 2024-05-08 10:39:18