A Multiplexed Approach to Assess Small Cell Lung Cancer Subtype Heterogeneity in Primary and Patient-Derived Tumor Samples
- Author(s)
- Ng, J; Pan, E; Johnston, A; Ribera, NT; Kersbergen, A; Hess, JB; Best, SA; Tsui, E; Steinfort, D; Sutherland, KD;
- Journal Title
- Methods in Molecular Biology
- Abstract
- Unlocking the heterogeneity of cancers is crucial for developing therapeutic approaches that effectively eradicate disease. As our understanding of markers specific to cancer subclones or subtypes expands, there is a growing demand for advanced technologies that enable the simultaneous investigation of multiple targets within an individual tumor sample. Indeed, multiplex approaches offer distinct benefits, particularly when tumor specimens are small and scarce. Here we describe the utility of two fluorescence-based multiplex approaches; fluorescent Western blots, and multiplex immunohistochemistry (Opal™) staining to interrogate heterogeneity, using small cell lung cancer as an example. Critically, the coupling of Opal™ staining with advanced image quantitation, permits the dissection of cancer cell phenotypes at a single cell level. These approaches can be applied to patient biopsies and/or patient-derived xenograft (PDX) models and serve as powerful methodologies for assessing tumor cell heterogeneity in response to therapy or between metastatic lesions across diverse tissue sites.
- Publisher
- Springer Nature
- Keywords
- Humans; *Small Cell Lung Carcinoma/pathology/metabolism/diagnosis; *Lung Neoplasms/pathology/metabolism/diagnosis; *Immunohistochemistry/methods; Animals; Biomarkers, Tumor/metabolism; Mice; Genetic Heterogeneity; Blotting, Western/methods; Single-Cell Analysis/methods; Cell Line, Tumor; Akoya; Fluorescent Western Blots; Li-COR; Multiplex immunohistochemistry (Opal™); Small cell lung cancer (SCLC); Tumor heterogeneity
- Research Division(s)
- Advanced Technology And Biology; Personalised Oncology; Cancer Biology And Stem Cells
- PubMed ID
- 38676800
- Publisher's Version
- https://doi.org/10.1007/978-1-0716-3858-3_10
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-05-08 10:06:38
Last Modified: 2024-05-08 10:39:32